Deqor v3
Enter your sequence in FASTA or raw format here (5kb is the limit):
Genome Database:
H. sapiens mRNA (NCBI)
M. musculus mRNA (NCBI)
T. castaneum (Tcas5.2)
D. melanogaster mRNA (Flybase/RefSeq)
A. gambiae mRNA (ENSEMBL)
D. rario mRNA (zfin v9 ENSEMBL)
S. mediterranea mRNA (ab initio library Jochen Rink Lab)
DEQOR now uses
bowtie
to detect off-targets. Database indices will be slowly added. If you need to use a particular database urgently, please write surendra@mpi-cbg.de
Settings
siRNA effectiveness
GC Content
gcLower:
gcUpper:
gcPenalty:
Strand preference in RISC assembly
GC(5')/AT(3') antisense penalty:
GC/AT symmetry:
Show advanced settings
Polynucleotide Stretch
polNuclPenalty:
Check for toxicity
toxCheck:
Positional preferences
not
T
C
G
A
Deqor is developed jointly between the
Buchholz
and
Habermann
labs. For help/other questions, write surendra@mpi-cbg.de
Please cite : Henschel, A., et.al., Nucleic Acids Res. 2004 Jul 1;32(Web Server issue):W113-20
Ready to use esiRNAs can be obtained from Sigma Aldrich. To order go to